Julia H. Chariker1,2,
Eric C. Rouchka2,4,*
1Deparmtent of Psychological and Brain Sciences, University of Louisville.
2Kentucky Biomedical Research Infrastructure Network (KBRIN) Bioinformatics Core.
3James Graham Brown Cancer Center.
4Department of Computer Engineering and Computer Science, University of Louisville.
Analysis of g-quadruplex forming sequences (QFS) was performed on the hg38 assembly of the human genome to identify large scale distribution patterns. At a locus level, QFS are known to provide structural utility, including transcriptional and translational control. Chromosomal-level patterns of distribution were analyzed in order to further elucidate biological mechanisms operating at larger scales. Significant correlations were found between QFS on forward and reverse DNA strands at one megabase intervals, as well as within transcriptional units. Unique high density banding patterns were discovered across chromosomes with the highest density region occurring within four megabases of one end of most chromosomes. Visualization of the results indicated these regions likely have a high stabilization function during meiosis in agreement with previous studies for chromosomal homolog pairing.
Funding provided by National Institutes of Health (NIH) grants P20GM103436 (Nigel Cooper, PI) and P20GM106396 (Donald Miller, PI). The contents of this work are solely the responsibility of the authors and do not represent the official views of the NIH or the National Institute for General Medical Sciences (NIGMS).